FastQCFastQC Report
Mon 6 Feb 2017
SRR2079151_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079151_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3137568
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA34500.033.379511
GGTATCA17850.029.8357731
ATCAACG43300.025.3365273
TCAACGC42900.025.1706774
TATCAAC45450.024.309572
CAACGCA44700.024.0802975
AACGCAG46700.023.2706476
GTACATG64850.020.2108251
TACATGG65100.019.570712
ACGCAGA54800.019.0116757
ACATGGG65300.018.4910983
CGCAGAG56800.018.0995968
TAGCCCT19050.016.4798894
AGAGTAC56150.015.920320511
GTGTAGC17400.015.7011941
GAGTACT29650.015.59672412
CAGAGTA61000.015.16375410
TATACTG13600.014.9667635
CATGGGG45200.014.7308134
GTAATAC6400.014.5543113
Produced by FastQC (version 0.11.5)