FastQCFastQC Report
Mon 6 Feb 2017
SRR2079151_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079151_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3137568
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22200.040.502891
GTATCAA54900.038.1108551
ATCAACG77800.027.4188753
TCAACGC77550.027.3737354
TATCAAC77550.027.0176542
CAACGCA81000.026.0799755
AACGCAG84700.024.9846486
ACGCAGA97900.021.0802697
CGCAGAG100050.020.4843968
AGAGTAC96450.019.53157611
GTACATG65100.019.4431111
GAGTACT71500.018.8676412
CAGAGTA102400.018.80100810
TACATGG64500.018.4093932
GCAGAGT107250.018.079489
AGTACTT77100.017.71843713
GTACTTT77750.017.61804814
GTATAGA7750.017.403181
ATACCGT3205.456968E-1217.261016
ACATGGG69200.017.0592823
Produced by FastQC (version 0.11.5)