FastQCFastQC Report
Mon 6 Feb 2017
SRR2079150_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079150_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2944923
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA36850.033.041751
GGTATCA19950.028.9555241
TCAACGC44350.027.1479534
ATCAACG44800.027.0292783
CAACGCA45350.026.7779945
TATCAAC46750.025.7005542
AACGCAG48600.025.1292576
ACGCAGA56700.021.1735697
CGCAGAG59400.020.1530578
GTACATG65350.019.0552561
TACATGG64350.018.7786482
AGAGTAC55350.018.7701711
CAGAGTA61200.017.59634410
ACATGGG67350.017.3647043
GCAGAGT64500.017.337939
GTATAGA7800.015.5214151
CGTATGA2051.4531048E-515.13660156
CATGGGG48800.015.1286174
GAGTACT28350.014.73015612
GTGTAGC14150.014.6673831
Produced by FastQC (version 0.11.5)