FastQCFastQC Report
Mon 6 Feb 2017
SRR2079150_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079150_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2944923
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA57100.040.1527521
GGTATCA23100.036.527151
ATCAACG77150.029.5706963
TATCAAC77600.028.9989242
TCAACGC78300.028.7837564
CGCGACG605.4296316E-428.74511152
CAACGCA80800.027.594165
AACGCAG84100.026.1910866
TATACCG1253.4939367E-622.0889975
ACGCAGA99350.021.8506347
CGCAGAG103550.020.8615468
GAGTACT69750.020.23422612
AGAGTAC97600.020.15266811
GTACATG66050.019.371661
AGTACTT75250.018.88678713
CAGAGTA105000.018.86383810
GCAGAGT110450.018.4329059
GTACTTT78000.018.4031214
TACATGG66650.018.3315472
ACATGGG70200.017.453693
Produced by FastQC (version 0.11.5)