FastQCFastQC Report
Mon 6 Feb 2017
SRR2079149_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079149_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences998893
Sequences flagged as poor quality0
Sequence length75
%GC36

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA90480.9058027236150419No Hit
GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGAATTAATTGAAATTTTATGTTGAGCT19920.19942075877996943No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17790.1780971535489787No Hit
TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGAA17020.17038862020256423No Hit
GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAACTAAAAGAATTACAGCTAGAAACCCC16670.1668847414087395No Hit
CTATTAAAGGTTTTTTCCGTTCCAGAAGAGCTGTCCCTCTTTTGGCTATAATCTAAACTTACTTTTTGATTTTGT15430.15447099939633174No Hit
GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGTGAGCTTTTTAGTTTGTGTCGGAAGC14670.14686257687259796No Hit
GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAGCTCGTTAGGCTTTTCACCTCTACCT14210.14225747902928543No Hit
ATTTTAAACTTGCTAAAAAAACAACAAAATCAAAAAGTAAGTTTAGATTATAGCCAAAAGAGGGACAGCTCTTCT13610.13625082966844299No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAAC13240.13254672922925678No Hit
AATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAGTTTAAAATTGAACTTAAATTCATTTTTTGGGTAA13240.13254672922925678No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACTGCAAATTCTAGAATTGTGAGTAGAA11940.11953232228076481No Hit
CTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTAATTAAAAGTAAGAACAAGCAAAGATTAAACCTTGTAC11800.11813077076323489No Hit
GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAATATGATTAGGGAAACAATTATTAGGG11480.11492722443745226No Hit
GAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11390.1140262270333259No Hit
GCTATCACCAAGCTCGTTAGGCTTTTCACCTCTACCTAAAAATCTTCTCACTATTTTGCCACATAGACGAGTTGA11230.11242445387043457No Hit
TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATGGCATTAGCAGTCCGGCTTACAGCTA11200.11212412140239245No Hit
CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCACTATGAGCTGGAGCCGTAATTACAG11190.11202401057971173No Hit
CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTATGCCTGGAGAATTGGAATTCTTGTTA11150.11162356728898891No Hit
CCAATAAAGAAAGCGTTCAAGCTCAACATAAAATTTCAATTAATTCCATAATTTACACCAACTTCCTAAACTTAA10800.10811968849516415No Hit
CCTTTAATAGTGAATAATTAACAAAACAGCTTTTAACCATTGTAGGCCTAAAAGCAGCCACCAATAAAGAAAGCG10580.10591725039618859No Hit
GAATAGGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTATTATTGTTGGGGTAATGAATGAGGC10490.1050162529920622No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTACG350.001349359239.41890333
GTATCAA26250.033.214991
CCTTTAG2550.032.5637131
CGGGAGT652.3195491E-531.84950646
GGTATCA12450.031.680961
GTATGGT2100.031.303811
CTTTAGG2450.031.0108092
CTTCGCA450.00461549830.67603913
AGCGACT450.00461549830.67603912
TCGCAGG450.004616629330.67450315
AACGCAT450.00462342230.6652876
CATTCCG2500.030.369289
GGCATTC2450.029.5686177
ACCGATT605.437445E-428.73575459
GCGTGTA1950.028.29366759
CGTCTTA1601.8189894E-1228.03841415
TCCGGTG3000.027.60843512
CGTTGTC500.0077399227.59599542
TGCGTGT2000.027.58632558
TAGGGTC1900.027.2356174
Produced by FastQC (version 0.11.5)