FastQCFastQC Report
Mon 6 Feb 2017
SRR2079148_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079148_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33105384
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGAT38500.014.42410851
TTGCGCG20100.011.3260128
ACACGCG25800.010.16188369
CGCGTTC25250.09.97365314
TTAGCGA18300.09.61261752
TCGCACG17550.09.2373619
GACGATC67450.09.1024982
CGCCAAC85250.08.9417341
TACGTCG20200.08.36975948
CACGATA35850.08.18095342
CTTAGCG26600.08.1692611
CGTTCGC33700.08.08704716
ACGATCC47800.08.0818613
GTCGCAC23100.08.0632648
CTTGAAT123150.08.0104241
GCGTTCG33050.07.932953415
CCGGAAT82200.07.93074231
CCGAAAT50900.07.92851641
CGACAAT30950.07.9126431
TACCGGT51050.07.772753744
Produced by FastQC (version 0.11.5)