FastQCFastQC Report
Mon 6 Feb 2017
SRR2079148_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079148_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33105384
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG34000.033.06585748
TATCTCG47350.023.74112741
TCGTATG51250.022.81010245
CGTATGC58450.019.1743946
ACCGCTC61150.018.8363632
TCACCGC66900.016.90817330
CTCGTAT69250.016.3331644
ATGCCGT77950.014.55535649
ACGATAC47400.014.54998660
GTATGCC81450.014.14166147
CACCGCT86500.013.83446431
GTCACCG83050.013.74482729
TACGATA51100.013.49668859
TGCCGTC86450.013.48325550
TCTCGTA86300.013.38559643
CGCTCAT88050.013.31669834
CTCGAAT109850.013.3049741
CATACGA52000.013.06416557
GCCGTCT89700.012.84054151
ACACGTC94150.012.70923513
Produced by FastQC (version 0.11.5)