FastQCFastQC Report
Mon 6 Feb 2017
SRR2079147_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079147_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33960624
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGCG71150.022.59209410
GATCGGG84300.018.1259651
ATCGGGA90400.016.5211892
CGGGGTA27950.015.8015739
TTGCGCG19600.014.7826638
ACGGCCC69150.014.36920533
CGACGAT36750.014.3601521
GGGGACG91550.013.3413729
TCGGGGG79500.012.49881150
GGGGCGT54650.012.49832411
GACGGCC93600.012.42196732
TCGGGAG126300.011.9616733
TTAGCGA16750.011.9435922
CGGGTTA23100.011.65006861
TCGCACG24100.011.5929929
CGGGTAG34600.011.56567215
GGCGGGT55400.011.14632213
CCGGGGT67700.011.110661538
GGGGGGC149850.011.05116355
GGGGGCG102800.010.97433356
Produced by FastQC (version 0.11.5)