FastQCFastQC Report
Mon 6 Feb 2017
SRR2079147_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079147_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33960624
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA608790.17926349056483767TruSeq Adapter, Index 27 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG86050.056.02730648
TTACTCG93300.052.0355935
TACTCGA103850.046.64967736
TCGTATG108150.045.15086745
ACTCGAT108300.044.51045637
ATTACTC116300.041.90026534
CGTATGC117600.041.05385246
ATGCCGT130750.037.00502449
GTATGCC138200.035.26002547
CTCGTAT132350.035.17494644
ACATTAC140950.034.76828832
TCTCGTA133600.034.5597243
GCCGTCT141350.034.30094551
TGCCGTC142050.034.2069650
CATTACT146800.033.38276733
CGATCTC158650.029.8634240
CCGTCTT167900.028.97963352
ATCTCGT161950.028.42473242
CGTCTGA176600.027.7468416
TCGATCT170200.027.35001439
Produced by FastQC (version 0.11.5)