FastQCFastQC Report
Mon 6 Feb 2017
SRR2079146_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079146_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28757589
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGAT34150.011.413291
CGCCCGT32500.09.76642336
TTGCGCG12350.09.4958448
CGCGAAT15750.09.20100748
TTAGCGA12350.08.9372812
CGAAGAT84750.08.668881
CTCGAAT82300.08.6335731
TCGCCCG53850.08.58510735
AGGGGCG53350.08.53473110
ACGATCC37000.08.2035353
CGAGAAT78950.08.1698231
CGAATCG49200.08.13475424
CGAGAAC99050.08.0789811
GACGATC50150.07.9782832
CGGAAAT51550.07.96235281
CTCAAAT106450.07.9061911
TCGCACG14400.07.90442049
CGAAAAT64300.07.8855041
TTCGCGT12053.6379788E-127.729832668
TACGGCG19200.07.7247759
Produced by FastQC (version 0.11.5)