FastQCFastQC Report
Mon 6 Feb 2017
SRR2079146_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079146_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28757589
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG39000.038.2011648
TCGTATG49050.031.42746445
CGTATGC56450.026.51360946
CTCGTAT71950.020.60980844
ATGCCGT78750.019.13770349
GTATGCC80800.019.03636247
AATCTCG78000.018.74487941
TCTCGTA81150.018.3146843
GCCGTCT88150.017.09594751
TGCCGTC90500.016.5767650
CTCGAAT93850.014.2787891
ATCTCGT103850.014.27817142
ACGATAC31350.013.74942860
CCGTCTT112900.013.53143352
CTCCGAT77950.013.3266131
CGATTAG46700.012.6778884
CATACGA35650.012.67166657
CTCGTTT80000.012.5089971
GTCGGTT47350.011.9201661
TACGATA37200.011.680160559
Produced by FastQC (version 0.11.5)