FastQCFastQC Report
Mon 6 Feb 2017
SRR2079145_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079145_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32570362
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGAT37000.012.4920051
ACGATAC33250.08.92514543
CACGATA32550.08.90455342
TTGCGCG16500.08.7800528
GACGATC59100.08.4626732
CCGCTAA34500.08.20081928
ACGATCC44000.08.0744123
CGATACA40050.08.01238144
ACACGCG19150.07.92627269
CGACAAT33400.07.74540041
TCGCACG16100.07.7126759
CGAGAAC111900.07.6445281
AGGGGCG53600.07.59398310
CTTGAAT119650.07.49531271
CGCGTTC22200.07.4589914
TTCGATC24050.07.4578792
CTCGAAT92600.07.44985961
CCGGTAT33450.07.427309546
CGAATCG52000.07.365022724
CGCGCTA17350.07.3558139
Produced by FastQC (version 0.11.5)