FastQCFastQC Report
Mon 6 Feb 2017
SRR2079145_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079145_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32570362
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG36750.034.344948
TCGTATG50050.026.93984845
CGTATGC56200.022.70345346
ACGAGAT57100.022.4067232
CGAGATT62900.020.28577233
CACGAGA74200.018.03306831
CTCGTAT72350.017.87348444
TCACGAG75150.017.71332630
ATGCCGT80400.016.29921549
GTATGCC81700.015.87116947
TCTCGTA85250.015.04680643
AGTCACG91400.014.60187228
GCCGTCT91700.014.02684451
TGCCGTC94750.013.97626550
ACGATAC36300.013.67906260
GTCGGTT56200.013.3742221
GTCACGA99300.013.19708829
ATCTCGT103350.012.44497942
CATACGA40600.012.23059557
CTCGAAT112950.011.6837921
Produced by FastQC (version 0.11.5)