FastQCFastQC Report
Mon 6 Feb 2017
SRR2079144_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079144_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18095794
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGCG59000.032.97476210
GATCGGG67650.031.6127951
ATCGGGA69050.030.8217162
GGGTATA41500.029.9283134
TCGGGGG65400.026.32501250
CGGGTAG26100.025.51024215
GGCGGGT41250.021.91165213
GGGGATA53450.021.7525434
AGGGTAT56300.021.32541533
GGTATAG32600.020.9546435
GTAGGGG67450.020.76507418
TCGGGAG89050.020.6068043
TAGGGGA69450.019.52155919
CGGGTTA15850.019.37321761
GCGGGTA28700.019.35270914
GGGGCGT41100.018.71774911
CGGGAGA109600.017.7499834
AGGGGAA156550.017.65107720
GGTATAA41450.016.4806135
CGGGGGG107700.015.79292751
Produced by FastQC (version 0.11.5)