FastQCFastQC Report
Mon 6 Feb 2017
SRR2079144_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079144_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18095794
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA807690.44634128792580197TruSeq Adapter, Index 2 (97% over 37bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA236800.13085913776427827TruSeq Adapter, Index 2 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG143700.047.8306948
TCGTATG150150.045.9123145
TATCTCG149600.045.57106841
CGTATGC152300.045.15145546
CTCGTAT161350.042.5320444
ATGCCGT162450.042.24661349
TGCCGTC170500.040.49469850
GTATGCC171600.040.41644347
TCTCGTA169450.040.2937543
ACCGCTC171950.040.09293732
GCCGTCT172050.039.8677651
TCACCGC176800.039.16887730
ATCTCGT178450.038.30021342
CGCTCAT180650.038.18117534
CACCGCT183250.037.7337631
CCGTCTT185150.037.15873352
GTCACCG187700.036.8762229
CCGCTCA191000.036.14830833
TTATCTC193500.035.49873440
CATTATC201000.034.17339738