FastQCFastQC Report
Mon 6 Feb 2017
SRR2079143_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079143_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30404066
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN893320.29381596527253956No Hit
GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC353980.1164252176008301No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTTTG380450.026.2687571
TGTTTGA374950.025.9511992
GTTTGAG431400.019.0320341
TTTGAGG276000.017.2329832
TTGAGGG190450.015.7071563
CGTACAT32950.015.47542119
AATTCGT35800.015.46542715
CGTCTTA85800.014.69836615
AAATTCG38650.014.15479814
TCGTACA37200.014.11789318
TTTGACG40600.013.9760934
CGCAATA61050.013.81900936
GTATTAG69900.013.5323471
CGCGATC12700.013.50116823
CTAATCG53500.013.18746119
TAGGGTC102900.013.1447354
GTATTGG66300.012.7670851
ATACGAA66000.012.66818740
GTCCTAT76850.012.4523291
ATAGGGT109250.012.4424723
Produced by FastQC (version 0.11.5)