FastQCFastQC Report
Mon 6 Feb 2017
SRR2079143_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079143_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30404066
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC357570.11760598072639364No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTTTG361800.025.580381
TGTTTGA362850.024.9297832
GTTTGAG420850.018.9616891
TTTGAGG264350.016.967922
CGTCTTA86400.015.53571315
TTGAGGG182500.015.40039353
AATTCGT33150.014.47065215
CGCGCAT18950.014.39112340
TTTGACG40450.014.3099984
CGTACAT33450.014.07745619
TCATACG32500.013.87202438
TACGGAC68600.013.75322843
CGCAATA60000.013.63619336
AAATTCG35650.013.51891614
CGTATCG17150.013.4750053
CGCGATC11600.013.46297723
ACGACCG72900.013.3118717
TAGGGTC107700.013.2422474
ACCGCTA71850.013.22288838
GTATTAG68950.013.09380151
Produced by FastQC (version 0.11.5)