FastQCFastQC Report
Mon 6 Feb 2017
SRR2079142_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079142_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27532412
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN809610.29405705537168336No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTTGA335200.025.5952632
GTGTTTG358150.024.799331
GTTTGAG393250.018.5283641
TTTGAGG253150.016.1982062
TTGAGGG174850.014.8563333
TTTGACG34950.014.158534
TACGGAC46250.013.87075443
CGCGATC9100.013.76894923
ACCGCTA47050.013.62380238
CGTCTTA47600.013.33925915
CTAATCG44450.013.05438919
AATTCGT24750.012.51655215
CGCTACG52050.012.46071340
TAATCGA47550.012.389914520
ACGACCG54900.012.28605517
CGCAATA50350.012.11922236
CGTACAT25800.012.09742819
GTCCTAT69800.012.0946921
GTATTAG58750.012.0372971
AAATTCG26000.012.00002214
Produced by FastQC (version 0.11.5)