FastQCFastQC Report
Mon 6 Feb 2017
SRR2079142_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079142_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27532412
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTTGA331650.023.9292772
GTGTTTG342500.023.6383251
GTTTGAG377550.018.038031
CGCGATC9150.015.86632223
TTTGAGG240600.015.6965982
CGTATCG14400.014.977783
TTGAGGG163050.014.83408453
CGTCTTA47550.014.62369915
TTTGACG34250.014.4579994
TACGGAC44400.013.37369343
CGCAATA43650.012.69727636
ACCGCTA48300.012.4757838
ACGACCG51100.012.40242317
CCGCGAT12050.012.23048122
GTTTGAC149200.011.9481333
CGCGCAT15200.011.71985640
CGCTACG51850.011.66446340
ACCGATT54750.011.65538720
CCGATTT55150.011.64726421
TTCGGCA36600.011.35729633
Produced by FastQC (version 0.11.5)