FastQCFastQC Report
Mon 6 Feb 2017
SRR2079141_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079141_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26070383
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN752470.2886302053943741No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTTGA314900.025.5642812
GTGTTTG328000.025.3028641
GTTTGAG374250.018.726991
TTTGAGG242100.016.4849012
TTGAGGG165050.015.1667773
CGCAATA49050.014.36938436
TTTGACG33000.013.5965344
CGTCTTA46100.013.44054715
CTAGCGG54650.013.21121129
TACGGAC48300.013.14509643
GTCCTAT73100.013.0617071
ACCGCTA50900.012.98094338
CAATACG55700.012.81128238
ATACGAA56500.012.68928440
ACGACCG53700.012.60255517
GTATTAG53650.012.5650341
CGGCGCA64750.012.54206633
CTAATCG42300.012.52287419
AATTCGT23850.012.06845515
AATACGA59300.012.05334739
Produced by FastQC (version 0.11.5)