FastQCFastQC Report
Mon 6 Feb 2017
SRR2079141_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079141_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26070383
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTTGA298600.024.2924842
GTGTTTG313800.023.7668691
GTTTGAG355900.018.4063931
TTTGAGG229050.016.5550122
TTGAGGG153050.015.2134213
CGTCTTA49100.014.92303815
CGCGATC10850.014.39413523
ACACCGT39200.013.5861226
CGTATCG13200.013.33799653
TTTGACG31950.013.2941884
CGCAATA46900.013.27169636
AATTCGT23400.013.25860215
ACGACCG53500.012.79144117
CCGCGAT12250.012.74911622
CGTACAT24800.012.68780119
TACGGAC50700.012.66645943
AAATTCG25400.012.56243414
ACCGCTA53450.012.42621138
CTAATCG32500.012.25454619
CCGATTT57950.012.03336221
Produced by FastQC (version 0.11.5)