FastQCFastQC Report
Mon 6 Feb 2017
SRR2079140_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079140_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29330905
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN860610.2934140627437169No Hit
GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC535580.1825992072184612No Hit
CTTTATTGGTGGCTGCTTTTAGGCCTACAATGGTTAAAAGCTGTTTTGTT420970.1435243815354487No Hit
CTATTAAAGGTTTTTTCCGTTCCAGAAGAGCTGTCCCTCTTTTGGCTATA390270.13305760596203903No Hit
CCATAGGGTCTTCTCGTCTTATTATTATATTTCAGCCTCTTCACTGAAAG364940.12442166377068829No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTTTG387850.027.3526541
TGTTTGA381650.027.2302932
GTTTGAG471200.019.9767281
CGTACAT45600.019.4249819
TCGTACA46500.019.13921218
AATTCGT47850.018.91706515
TTTGAGG287400.018.7613562
TTGAGGG199250.017.5527233
AAATTCG51650.017.48368814
CGTCTTA112400.015.7741415
GTATTGG81400.015.0963651
TTTGACG43250.014.7978584
CGCAATA65200.014.66193836
TCTCGTC129650.013.9458712
TAGGGTC130950.013.8560824
GTATTAG81150.013.8355751
GCTATAG66850.013.76487632
CAATACG70650.013.56114838
TACGGAC61650.013.55269143
ATACGAA71050.013.44387940
Produced by FastQC (version 0.11.5)