FastQCFastQC Report
Mon 6 Feb 2017
SRR2079140_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079140_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29330905
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC536630.18295719139931071No Hit
CTTTATTGGTGGCTGCTTTTAGGCCTACAATGGTTAAAAGCTGTTTTGTT447710.15264104534108308No Hit
CTATTAAAGGTTTTTTCCGTTCCAGAAGAGCTGTCCCTCTTTTGGCTATA414410.14128783274842696No Hit
CCATAGGGTCTTCTCGTCTTATTATTATATTTCAGCCTCTTCACTGAAAG382990.13057558230814903No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTTTG358850.026.6243061
TGTTTGA358450.026.1874122
CGTACAT44100.020.40760619
GTTTGAG438500.019.6490631
AATTCGT46900.019.28360415
TCGTACA46900.019.00264218
AAATTCG48850.018.51601614
TTTGAGG272200.018.386732
TTGAGGG190150.017.5933863
TTTGACG36900.016.223564
CGTCTTA116300.015.93131315
CGCGATC8050.015.02849923
TCATACG43700.014.69419538
GTATTGG79950.014.4315981
TACGGAC66950.014.22300343
TCTCGTC131450.014.16320512
TAGGGTC133350.014.1281214
ACCGCTA68600.014.10499738
CGCAATA66500.014.08864936
GCTATAG66700.013.94886632
Produced by FastQC (version 0.11.5)