FastQCFastQC Report
Mon 6 Feb 2017
SRR2079139_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079139_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22106960
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN681900.30845489384338687No Hit
GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAACTAAAAGAATTACAGCTAGAAACCCC315810.142855462714005No Hit
CTATTAAAGGTTTTTTCCGTTCCAGAAGAGCTGTCCCTCTTTTGGCTATAATCTAAACTTACTTTTTGATTTTGT240820.10893401896959148No Hit
CTTTATTGGTGGCTGCTTTTAGGCCTACAATGGTTAAAAGCTGTTTTGTTAATTATTCACTATTAAAGGTTTTTT234040.1058671115341051No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTTGA393450.037.2483332
GTGTTTG401700.037.232551
GTTTGAG423750.026.7815341
TTTGAGG276000.023.184652
TTGAGGG190050.021.4513113
TTTGACG42200.019.9450514
CGTCTTA69000.018.77209915
TTGAGTG114950.016.6298585
GCGTAAA62450.016.111469
ACGACCG66200.016.0881217
GTTTGAT179300.015.9730891
GGCGTAA64000.015.77306368
TTGACGG32900.015.3401415
TCTCGTC88250.015.106712
ACCGATT71800.015.0405620
CCGATTT72600.014.83760621
TTCGCGA6100.014.69170149
TGAGGGT107500.014.6002654
TCGGACG72800.014.55548113
TAGGGTC94600.014.4033584
Produced by FastQC (version 0.11.5)