FastQCFastQC Report
Mon 6 Feb 2017
SRR2079139_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079139_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22106960
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAACTAAAAGAATTACAGCTAGAAACCCC317500.14361992784172947No Hit
CTATTAAAGGTTTTTTCCGTTCCAGAAGAGCTGTCCCTCTTTTGGCTATAATCTAAACTTACTTTTTGATTTTGT255030.11536185888968904No Hit
CTTTATTGGTGGCTGCTTTTAGGCCTACAATGGTTAAAAGCTGTTTTGTTAATTATTCACTATTAAAGGTTTTTT247580.11199187948048941No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTTGA351850.033.998662
GTGTTTG358650.033.8848231
GTTTGAG376650.025.6363371
TTTGAGG251800.021.553262
CGTCTTA76050.021.0922515
TTGAGGG170400.019.9315193
TTTGACG38750.018.170774
TCTCGTC97200.016.68267312
TAGGGTC101950.016.6568454
GCGTAAA61150.016.52850369
ACGACCG75850.016.50924117
CGCGATC9250.016.41159823
ATAGGGT107850.016.2576753
GGCGTAA62250.016.18145668
CATAGGG111450.015.9530332
CGTATCG18050.015.8714193
TCGCGCT10050.015.78783961
TTGACGG31100.015.65140155
TTCGTTT126450.015.52158728
GTCGGAC81600.015.34795512
Produced by FastQC (version 0.11.5)