FastQCFastQC Report
Mon 6 Feb 2017
SRR2079138_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079138_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29337917
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN858330.29256678311551565No Hit
GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC341280.11632727708650892No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTTGA367350.026.0638582
GTGTTTG379450.025.9813481
GTTTGAG432000.018.5227811
TTTGAGG273200.016.862652
CGTACAT30500.015.85273919
TTGAGGG193500.015.3351373
AATTCGT31300.015.230762515
TTTGACG39050.014.3623364
CGTCTTA76850.014.1789515
TCGTACA34250.014.11383918
AAATTCG34100.013.98057514
TACGGAC59350.013.56012643
GTATTAG67850.013.4875141
ACCGCTA61450.013.47792638
CGCAATA53800.012.611238536
TCATACG29850.012.54474238
ACGACCG68200.012.402143517
GTTTGAT168350.012.3816921
TTGAGTG111800.012.3151155
GTATTGG69700.012.273251
Produced by FastQC (version 0.11.5)