FastQCFastQC Report
Mon 6 Feb 2017
SRR2079138_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079138_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29337917
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC342040.11658632751602645No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTTGA344100.024.8807162
GTGTTTG356250.024.5950781
GTTTGAG399450.018.7543091
TTTGAGG256300.016.8823492
CGTACAT30050.015.816195519
AATTCGT31300.015.536345515
AAATTCG31500.015.36910514
TTGAGGG183200.015.2212093
CGTCTTA80350.015.0618315
TACGGAC64850.014.48061643
ACCGCTA69200.013.79304638
ACGACCG71200.013.56760717
TCGTACA35450.013.46985518
TCATACG32700.013.38387538
CGCAATA52300.013.37315336
TTTGACG36950.012.9851414
GTATTGG62450.012.9055881
CGCGCAT17650.012.83442640
CGCTACG75100.012.65108540
TACGATT35550.012.620440541
Produced by FastQC (version 0.11.5)