FastQCFastQC Report
Mon 6 Feb 2017
SRR2079137_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079137_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29242528
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN858300.2935108756671106No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTTGA358700.025.7719422
GTGTTTG369700.025.7246571
GTTTGAG414500.018.3746221
TTTGAGG264450.016.1973842
TTTGACG38700.014.8329824
TTGAGGG179550.014.7255123
CGTACAT26200.014.42853319
AATTCGT27550.014.27480215
AAATTCG28200.013.94577114
CGTCTTA55950.013.78306115
TACGGAC59800.013.4566843
ACCGCTA61300.013.33102538
ACGACCG63650.013.15153517
CTAATCG54100.013.00013119
GTATTAG63600.012.9622211
CGCAATA56750.012.92580836
TCGTACA30000.012.74495918
CGCTACG65400.012.3840
CGAAACG38250.012.29638219
GTTTGAC183050.012.2328993
Produced by FastQC (version 0.11.5)