FastQCFastQC Report
Mon 6 Feb 2017
SRR2079137_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079137_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29242528
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTTGA346400.024.4234732
GTGTTTG358850.024.2322241
GTTTGAG398100.018.3362461
TTTGAGG255550.015.8985592
CGTCTTA60550.015.66273915
TACGGAC59350.014.00674443
CGTATCG17100.013.9004733
TTGAGGG179650.013.7457233
TTTGACG37150.013.7444974
CTAATCG44800.013.359570519
ACCGCTA62750.013.10735538
CGCGCAT17500.013.06986340
ACGACCG65850.012.96579517
CGCAATA54550.012.3775836
ACACCGT43750.012.37496956
CCGATTT69150.012.374475521
TAGGGTC81750.012.3034854
ACCGATT69950.012.2995920
TCTCGTC78850.012.25184812
CGCTACG66900.012.19617240
Produced by FastQC (version 0.11.5)