FastQCFastQC Report
Mon 6 Feb 2017
SRR2079136_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079136_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26791847
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN785120.293044372789976No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTTGA328150.025.389032
GTGTTTG341350.025.2967431
GTTTGAG373000.017.8115311
TTTGAGG242050.015.478642
TTGAGGG168700.013.729893
CGCAATA42750.013.70605736
CGTACAT22000.013.38764819
CTAATCG44500.013.13843919
TTTGACG36100.013.0381094
TACGGAC47700.012.61747143
GTATTAG60250.012.5088811
CTAGCGG48050.012.414371529
TAATCGA47100.012.36860220
AATTCGT24200.012.34708615
ACCGCTA48750.012.24441438
GTTTGAC151250.012.1580713
GGGTATA75300.012.1514041
CAATACG47950.011.98933838
CGTCTTA39850.011.90875415
TCGTACA25700.011.88495918
Produced by FastQC (version 0.11.5)