FastQCFastQC Report
Mon 6 Feb 2017
SRR2079136_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079136_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26791847
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTTGA309000.024.2876532
GTGTTTG327750.023.676361
GTTTGAG354850.018.0455991
TTTGAGG230850.015.7496092
TTGAGGG157700.014.8909483
CGTCTTA45550.014.25052415
TACGGAC48350.013.96414843
ACGACCG50900.013.96367517
CGTACAT22750.013.83089219
CGTATCG12550.013.6785643
ACCGCTA50300.013.6415638
AATTCGT23700.013.55506615
CGCGCAT14100.013.41425240
TTTGACG29750.013.1680864
CGCTACG53000.012.86390840
CCGATTT55950.012.81717121
CGCAATA42900.012.61184836
ACCGATT57000.012.54596420
ACACCGT36750.012.4562566
GTATTAG58450.011.8295121
Produced by FastQC (version 0.11.5)