FastQCFastQC Report
Mon 6 Feb 2017
SRR2079135_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079135_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33629788
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN993280.2953571993971535No Hit
GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC647530.1925465602102517No Hit
CTTTATTGGTGGCTGCTTTTAGGCCTACAATGGTTAAAAGCTGTTTTGTT478850.14238864663672576No Hit
CTATTAAAGGTTTTTTCCGTTCCAGAAGAGCTGTCCCTCTTTTGGCTATA475820.14148765969027222No Hit
CCATAGGGTCTTCTCGTCTTATTATTATATTTCAGCCTCTTCACTGAAAG464270.13805320449834535No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTTTG471500.028.3630261
TGTTTGA462100.028.1936822
GTTTGAG582850.019.8194451
TTTGAGG346000.019.4247072
CGTACAT49250.019.19004819
TCGTACA49950.018.91672718
AATTCGT52650.018.0270615
TTGAGGG238700.017.858593
AAATTCG53850.017.6258714
TTTGACG45100.017.1167644
TCATACG50800.015.43500338
GTATTGG88600.015.2934561
CGTCTTA145800.014.84288115
ATACGAT55200.014.06716440
TACGATT57050.013.98848241
TAGGGTC161600.013.8955434
CATACGA57900.013.7540239
TTGAGTG132800.013.5636015
TCTCGTC160300.013.554152512
ATAGGGT168100.013.4907693
Produced by FastQC (version 0.11.5)