FastQCFastQC Report
Mon 6 Feb 2017
SRR2079135_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079135_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33629788
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC631880.18789294776404777No Hit
CTTTATTGGTGGCTGCTTTTAGGCCTACAATGGTTAAAAGCTGTTTTGTT518510.15418176290614738No Hit
CTATTAAAGGTTTTTTCCGTTCCAGAAGAGCTGTCCCTCTTTTGGCTATA495470.147330693847966No Hit
CCATAGGGTCTTCTCGTCTTATTATTATATTTCAGCCTCTTCACTGAAAG485190.14427388004943711No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTTGA412400.027.185842
GTGTTTG427450.026.8921931
GTTTGAG529400.020.4654921
CGTACAT48400.019.68539819
TCGTACA48650.019.5399418
TTTGAGG318800.019.3718812
AATTCGT50700.018.70565415
AAATTCG51550.018.44138714
TTGAGGG221500.017.5377313
TTTGACG44850.016.6845724
CGTCTTA147050.016.400215
GTATTGG80950.015.4787691
TCATACG53950.015.04214738
TAGGGTC165000.014.9127054
TCTCGTC165200.014.71876312
CATAGGG179200.014.4803242
ATAGGGT172650.014.3922873
CGTATCG26150.014.1396953
CCATAGG185000.013.9269221
GTCTTAT177050.013.77059116
Produced by FastQC (version 0.11.5)