##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2079135_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 33629788 Sequences flagged as poor quality 0 Sequence length 50 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.429951922385 33.0 31.0 34.0 29.0 34.0 2 31.52674328485211 34.0 31.0 34.0 28.0 34.0 3 31.610507059990983 34.0 31.0 34.0 28.0 34.0 4 35.0684505653143 37.0 35.0 37.0 32.0 37.0 5 34.8014316950199 37.0 35.0 37.0 32.0 37.0 6 34.75249460389105 37.0 35.0 37.0 32.0 37.0 7 34.59717016354667 37.0 35.0 37.0 32.0 37.0 8 34.55540121156874 37.0 35.0 37.0 32.0 37.0 9 36.180414012719915 39.0 37.0 39.0 32.0 39.0 10 36.10095725254052 39.0 37.0 39.0 32.0 39.0 11 36.09254940887525 39.0 37.0 39.0 32.0 39.0 12 36.021436471737495 39.0 37.0 39.0 31.0 39.0 13 36.012645574810044 39.0 37.0 39.0 31.0 39.0 14 37.29319167875813 40.0 38.0 41.0 31.0 41.0 15 37.30276242003072 40.0 38.0 41.0 31.0 41.0 16 37.29022148459574 40.0 38.0 41.0 31.0 41.0 17 37.25616938173978 40.0 38.0 41.0 31.0 41.0 18 37.22141168418903 40.0 38.0 41.0 31.0 41.0 19 37.26024627333363 40.0 38.0 41.0 31.0 41.0 20 37.21060855334562 40.0 38.0 41.0 31.0 41.0 21 37.178421671882084 40.0 38.0 41.0 31.0 41.0 22 37.155042131101155 40.0 38.0 41.0 31.0 41.0 23 37.11081235480878 40.0 38.0 41.0 31.0 41.0 24 37.08259088638918 40.0 38.0 41.0 31.0 41.0 25 37.04748415303718 40.0 38.0 41.0 31.0 41.0 26 36.98795294219518 40.0 38.0 41.0 30.0 41.0 27 36.93366859761352 40.0 38.0 41.0 30.0 41.0 28 36.86657834417511 40.0 38.0 41.0 30.0 41.0 29 36.802750555549146 40.0 37.0 41.0 30.0 41.0 30 36.75214399210605 40.0 37.0 41.0 30.0 41.0 31 36.69197177216817 40.0 37.0 41.0 30.0 41.0 32 36.63450376196246 40.0 37.0 41.0 30.0 41.0 33 36.58126313493264 40.0 37.0 41.0 30.0 41.0 34 36.51912319518636 40.0 37.0 41.0 30.0 41.0 35 36.443599703929145 40.0 37.0 41.0 30.0 41.0 36 36.38615206257024 40.0 37.0 41.0 30.0 41.0 37 36.30588976653674 40.0 36.0 41.0 29.0 41.0 38 36.21751421686036 40.0 36.0 41.0 29.0 41.0 39 36.1592297281208 40.0 36.0 41.0 29.0 41.0 40 36.087976022923485 40.0 36.0 41.0 28.0 41.0 41 36.010803338992204 40.0 36.0 41.0 28.0 41.0 42 35.9095729060201 40.0 35.0 41.0 28.0 41.0 43 35.82044855590526 40.0 35.0 41.0 27.0 41.0 44 35.73808499774069 40.0 35.0 41.0 27.0 41.0 45 35.632647758588305 40.0 35.0 41.0 27.0 41.0 46 35.52205119461354 40.0 35.0 41.0 26.0 41.0 47 35.40776340903487 40.0 35.0 41.0 26.0 41.0 48 35.27080884363588 40.0 35.0 41.0 26.0 41.0 49 35.15528224560916 39.0 35.0 41.0 26.0 41.0 50 35.006328704778035 39.0 35.0 41.0 25.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.45657653762371453 1101 2 0.45059729477005916 1101 3 0.47086341316175506 1101 4 0.39862584769140597 1101 5 0.4877010645559423 1101 6 0.5332690370746249 1101 7 0.5941943170924873 1101 8 0.5848194359645831 1101 9 0.6858025492006803 1101 10 0.6690850526354808 1101 11 0.6819622623368531 1101 12 0.6881388265968411 1101 13 0.6772587736981492 1101 14 0.7911503566452041 1101 15 0.805595907466703 1101 16 0.8073930089131238 1101 17 0.8272589983629004 1101 18 0.8358101206449859 1101 19 0.840222895356348 1101 20 0.8336131539713634 1101 21 0.8374824656907691 1101 22 0.8309168274149812 1101 23 0.8025655613618028 1101 24 0.8418135786561578 1101 25 0.8134792572925633 1101 26 0.8236872987854937 1101 27 0.821165647961891 1101 28 0.8418502308657452 1101 29 0.8657132323042518 1101 30 0.8707438562073833 1101 31 0.8760494685222611 1101 32 0.8842711088623076 1101 33 0.8865256624247166 1101 34 0.9121558849251556 1101 35 0.8844893669213292 1101 36 0.8835234657153705 1101 37 0.9143808755038876 1101 38 0.8776225308145555 1101 39 0.9183850143029915 1101 40 0.9194618252460884 1101 41 0.9283396703003106 1101 42 0.9536813743746464 1101 43 0.9652570289382396 1101 44 0.9421713385801453 1101 45 0.9848116298360594 1101 46 0.9867891407213918 1101 47 1.003870672356605 1101 48 1.0260597111642156 1101 49 0.9971373517367965 1101 50 1.0493064098477305 1102 1 0.42436156831027105 1102 2 0.402654835447656 1102 3 0.40409616067135445 1102 4 0.36163282275174424 1102 5 0.42034920034548406 1102 6 0.460995973389565 1102 7 0.4949162547996764 1102 8 0.5211031412187026 1102 9 0.5768758577731461 1102 10 0.5788053699184346 1102 11 0.6164800443639464 1102 12 0.6254878933482857 1102 13 0.6342168919877196 1102 14 0.7534705683470051 1102 15 0.7333612820329023 1102 16 0.759652719538515 1102 17 0.7207792580130103 1102 18 0.7392680815211676 1102 19 0.7386656408023669 1102 20 0.7300186989291717 1102 21 0.7468589217385286 1102 22 0.7358131144626796 1102 23 0.7391661438480455 1102 24 0.756729260550081 1102 25 0.7629288660552049 1102 26 0.7144651525258539 1102 27 0.7514533721561776 1102 28 0.7540997845063799 1102 29 0.7552417882640654 1102 30 0.7625340227873849 1102 31 0.7732849422454464 1102 32 0.7941103061888768 1102 33 0.8034443569253256 1102 34 0.7656224078865961 1102 35 0.7805554188665838 1102 36 0.8195862537867598 1102 37 0.8402361874729962 1102 38 0.829481671879094 1102 39 0.838899209239365 1102 40 0.860807657134643 1102 41 0.8695386244155898 1102 42 0.8977306086110133 1102 43 0.8997024205383539 1102 44 0.8716930102824065 1102 45 0.9211750611417386 1102 46 0.8972462777066141 1102 47 0.9121762033877019 1102 48 0.9188670867491808 1102 49 0.9529484316765959 1102 50 0.9367589634487601 1103 1 0.31410921130240865 1103 2 0.3084834050093548 1103 3 0.3469572995922725 1103 4 0.2777698825003725 1103 5 0.34582638451228576 1103 6 0.39927978983295986 1103 7 0.4403897222794271 1103 8 0.4244623810120416 1103 9 0.47053705248199407 1103 10 0.4974549786123248 1103 11 0.5117184881511534 1103 12 0.490191648363151 1103 13 0.512762008502925 1103 14 0.5964262769518314 1103 15 0.5747135909111307 1103 16 0.5904021377308339 1103 17 0.5766769383673918 1103 18 0.593312749105948 1103 19 0.5881325633777053 1103 20 0.5950680595656976 1103 21 0.5845247013114943 1103 22 0.581463478633431 1103 23 0.5999088952373626 1103 24 0.6045592092540844 1103 25 0.5836135727347838 1103 26 0.610903248729791 1103 27 0.6321562474855611 1103 28 0.654701284070832 1103 29 0.6722780181580816 1103 30 0.6518524864036479 1103 31 0.6639031112404936 1103 32 0.6370014827736341 1103 33 0.6569564276691651 1103 34 0.6592218791153357 1103 35 0.6923650040750786 1103 36 0.6727702108961182 1103 37 0.7010422695389167 1103 38 0.7246611766344273 1103 39 0.7146048056788814 1103 40 0.7143020737354249 1103 41 0.7338191660013109 1103 42 0.7722298542057686 1103 43 0.7562399295685012 1103 44 0.7297615280181944 1103 45 0.7890306693191675 1103 46 0.7597964570658959 1103 47 0.7575197533313798 1103 48 0.7639090378624047 1103 49 0.8011356931939915 1103 50 0.8004463401899571 1104 1 0.2959378042963685 1104 2 0.3192491556640995 1104 3 0.318901064283569 1104 4 0.25738520504695117 1104 5 0.30972118180839914 1104 6 0.35362614708971307 1104 7 0.4142687733910506 1104 8 0.4217666606502206 1104 9 0.4820789480309742 1104 10 0.4866591725918781 1104 11 0.4767606902283177 1104 12 0.5109317211212812 1104 13 0.5191418144833548 1104 14 0.5932561482539782 1104 15 0.5612337873890425 1104 16 0.5730546825652709 1104 17 0.5875242187309695 1104 18 0.6042299841248564 1104 19 0.5883626956686214 1104 20 0.575942049480588 1104 21 0.5966690828075301 1104 22 0.5863489923693592 1104 23 0.5960547383424526 1104 24 0.5937522836933979 1104 25 0.6066933623032043 1104 26 0.6221530151538275 1104 27 0.6163198153832425 1104 28 0.6171946220101105 1104 29 0.6098565069127346 1104 30 0.5944229159640315 1104 31 0.6267660997655184 1104 32 0.6093918708352248 1104 33 0.6057214247302838 1104 34 0.5791707802369572 1104 35 0.6135274153891856 1104 36 0.6177786746775524 1104 37 0.6123189137540805 1104 38 0.6212609069564152 1104 39 0.6061586278462485 1104 40 0.6095167548236446 1104 41 0.6355111508255646 1104 42 0.6227967599083257 1104 43 0.609249582733149 1104 44 0.6017906889511195 1104 45 0.624574899235256 1104 46 0.6206870904233952 1104 47 0.621998451274834 1104 48 0.6608002406894258 1104 49 0.624210121053224 1104 50 0.6125991579529853 1105 1 0.2536186624118635 1105 2 0.22461531715819305 1105 3 0.2520389654346431 1105 4 0.21266161240879455 1105 5 0.280690308569973 1105 6 0.3160534444203478 1105 7 0.36308010120437473 1105 8 0.36533777578885207 1105 9 0.3999390860611598 1105 10 0.39900378914690293 1105 11 0.40642683046093 1105 12 0.41827900616202385 1105 13 0.40384067721510775 1105 14 0.48485431625486797 1105 15 0.486698010294468 1105 16 0.48770133024783746 1105 17 0.48729977017835324 1105 18 0.5086629036079344 1105 19 0.49648539163956684 1105 20 0.4874920853175553 1105 21 0.4686101411092878 1105 22 0.4695684177930417 1105 23 0.47799850187246307 1105 24 0.4985103182229693 1105 25 0.47343396595640996 1105 26 0.4869639063225577 1105 27 0.4973767600922514 1105 28 0.4838510776457028 1105 29 0.5047012451814155 1105 30 0.5018388120723927 1105 31 0.5086427599785708 1105 32 0.5430504701195318 1105 33 0.5418952226136824 1105 34 0.5394440117841057 1105 35 0.5655845149448382 1105 36 0.5575735344446642 1105 37 0.5694826845726979 1105 38 0.5881839078020477 1105 39 0.5840754722624837 1105 40 0.5781852818501463 1105 41 0.5797951369498406 1105 42 0.629947787063017 1105 43 0.6266191033559494 1105 44 0.6173042125078183 1105 45 0.6506005471430854 1105 46 0.6135999271865984 1105 47 0.5999239746403902 1105 48 0.6482741212387211 1105 49 0.6673378780319368 1105 50 0.6751243990256839 1106 1 0.14677759601621432 1106 2 0.14847969678756456 1106 3 0.14233860777217444 1106 4 0.14260202694060808 1106 5 0.18237879743278285 1106 6 0.21234597830995483 1106 7 0.30404271518393955 1106 8 0.2700219301494613 1106 9 0.29347247160718126 1106 10 0.2859059435168305 1106 11 0.3174801682563242 1106 12 0.321379790293264 1106 13 0.3293489806616421 1106 14 0.3563912229808608 1106 15 0.34601980373248864 1106 16 0.3158549701723743 1106 17 0.32060890156271427 1106 18 0.3692381332461707 1106 19 0.32487099112734086 1106 20 0.33701043184925794 1106 21 0.3383805408092968 1106 22 0.3431344676177872 1106 23 0.3307189903443515 1106 24 0.336014291156971 1106 25 0.3362462520302145 1106 26 0.35756959428387347 1106 27 0.3417657290862408 1106 28 0.3593978978990364 1106 29 0.3717890175161358 1106 30 0.3601227463615686 1106 31 0.3916827598694539 1106 32 0.3904658360817521 1106 33 0.37553215537773355 1106 34 0.3545913825128224 1106 35 0.3760473021182804 1106 36 0.4069406910355369 1106 37 0.41084271632230696 1106 38 0.3788068252393515 1106 39 0.3843133631893778 1106 40 0.39907157730738163 1106 41 0.42262838271255987 1106 42 0.42665925091851165 1106 43 0.4540582061980061 1106 44 0.4513924263059721 1106 45 0.4586323083742556 1106 46 0.4213293418457411 1106 47 0.4032317807425798 1106 48 0.4343839102279148 1106 49 0.42347355154808497 1106 50 0.470195905825328 1107 1 0.1253877337963445 1107 2 0.11849295776713831 1107 3 0.14721388986951567 1107 4 0.12578175876591757 1107 5 0.1307276734686127 1107 6 0.16568971832322177 1107 7 0.2321656207993641 1107 8 0.20821600471561652 1107 9 0.21943657826972895 1107 10 0.2431544529712184 1107 11 0.24026075353987153 1107 12 0.23926290783443704 1107 13 0.26581797795822837 1107 14 0.27889301049880544 1107 15 0.24811951393543552 1107 16 0.2622912792618308 1107 17 0.26553965363229537 1107 18 0.29209078651270204 1107 19 0.28375972625228485 1107 20 0.2975932284291005 1107 21 0.3023898449991691 1107 22 0.28982566801067833 1107 23 0.2906094554410217 1107 24 0.27993005248481495 1107 25 0.2854806803128085 1107 26 0.2722287552733036 1107 27 0.2795633177115491 1107 28 0.3000669068855757 1107 29 0.2777884338472063 1107 30 0.324120306679724 1107 31 0.3309955042084596 1107 32 0.3104815260330014 1107 33 0.3097187390036211 1107 34 0.291707847534461 1107 35 0.32483326449838756 1107 36 0.3174873884182219 1107 37 0.34355989782030605 1107 38 0.3284598590155454 1107 39 0.34641379071918266 1107 40 0.3246792523652502 1107 41 0.3173357949631992 1107 42 0.3337272003904417 1107 43 0.3308721648818036 1107 44 0.3359241374202355 1107 45 0.36342879010389595 1107 46 0.39000582774163917 1107 47 0.3978003821301641 1107 48 0.40052586984620575 1107 49 0.4090198998104171 1107 50 0.4043401263184663 1108 1 0.028491035184998736 1108 2 0.07578428170018725 1108 3 0.07553702387302863 1108 4 0.05803795378722043 1108 5 0.03900924822907825 1108 6 0.12088262204002831 1108 7 0.1749928235579361 1108 8 0.1645339375856949 1108 9 0.15104796390427566 1108 10 0.17006701720673334 1108 11 0.17667443780331382 1108 12 0.13866715037151067 1108 13 0.1614184258895648 1108 14 0.1964083475310261 1108 15 0.18034353440730655 1108 16 0.17320101904949325 1108 17 0.17089645313737378 1108 18 0.17528973363502587 1108 19 0.20115248630479243 1108 20 0.16929279482286574 1108 21 0.19057659277115135 1108 22 0.22244547089524502 1108 23 0.17581171379713822 1108 24 0.17816620015319984 1108 25 0.19646902034343583 1108 26 0.19691121092564856 1108 27 0.2022477005027028 1108 28 0.21166691085838352 1108 29 0.2123056100389391 1108 30 0.1999226106385592 1108 31 0.2206539270948369 1108 32 0.17799979336061256 1108 33 0.19861303453794932 1108 34 0.20623900038991394 1108 35 0.19797233850109563 1108 36 0.18891424010956115 1108 37 0.2202380736447438 1108 38 0.2045778330365522 1108 39 0.21618924016438967 1108 40 0.22762834578515623 1108 41 0.2097243667830142 1108 42 0.24970118561306265 1108 43 0.24785647040285141 1108 44 0.25232246721760987 1108 45 0.28220126259909506 1108 46 0.2583638677149551 1108 47 0.2336727295407215 1108 48 0.20093632721577137 1108 49 0.20356203681239293 1108 50 0.2510752818306301 1109 1 -0.014720319241018132 1109 2 0.009292498089493506 1109 3 0.022476924298270973 1109 4 0.0028190277829693855 1109 5 0.039993713655640306 1109 6 0.0624197449819377 1109 7 0.12432414240753076 1109 8 0.10566195692223346 1109 9 0.10258884515631195 1109 10 0.08008148364527301 1109 11 0.10875899627749419 1109 12 0.10068910512556783 1109 13 0.10017813354406968 1109 14 0.06889392788954041 1109 15 0.06771334024034559 1109 16 0.09093791604321666 1109 17 0.033690227102248116 1109 18 0.0714636211624935 1109 19 0.10505020968474099 1109 20 0.0703817417717616 1109 21 0.0885044163386155 1109 22 0.07027574127396718 1109 23 0.0553248495412717 1109 24 0.06810675617295914 1109 25 0.10333632806460002 1109 26 0.07271783336967985 1109 27 0.11134880818185167 1109 28 0.06578805691678724 1109 29 0.057242301405104 1109 30 0.04019267513824332 1109 31 0.07974949262320052 1109 32 0.06269919217301378 1109 33 0.05372315780712 1109 34 0.07257394846374865 1109 35 -0.00820196478564128 1109 36 -0.030620494511239826 1109 37 0.07611945434987888 1109 38 0.08161560333940088 1109 39 0.058646990150492684 1109 40 0.06499114950267426 1109 41 0.01475341087775206 1109 42 0.03486984152183226 1109 43 0.06498987726541827 1109 44 0.08725263708297604 1109 45 0.07611753332295734 1109 46 0.09622200967692862 1109 47 0.034715204768232866 1109 48 0.031590961089293046 1109 49 0.03167514043768449 1109 50 0.016867082306610826 1110 1 -0.23456536057086552 1110 2 -0.21919504794506395 1110 3 -0.188834875326215 1110 4 -0.17760131600699225 1110 5 -0.22058619693768833 1110 6 -0.24869345631708484 1110 7 -0.24225922044085024 1110 8 -0.31362592922984334 1110 9 -0.3722639605335587 1110 10 -0.304026028774139 1110 11 -0.3449242654963456 1110 12 -0.3094614063515948 1110 13 -0.34890552019903254 1110 14 -0.4068901715113924 1110 15 -0.4032644238626659 1110 16 -0.38470666411068066 1110 17 -0.3879721539515444 1110 18 -0.37094797004743896 1110 19 -0.3059641056863782 1110 20 -0.3314936044019561 1110 21 -0.4274044174854623 1110 22 -0.3204458004085282 1110 23 -0.3643540609166678 1110 24 -0.40768356002862305 1110 25 -0.43574749639160615 1110 26 -0.3690628879619027 1110 27 -0.4313295181646666 1110 28 -0.41828856016005034 1110 29 -0.4265323263412739 1110 30 -0.352982449393366 1110 31 -0.507015922330531 1110 32 -0.48053932702681124 1110 33 -0.4663169312801685 1110 34 -0.3236318110899461 1110 35 -0.3180434932612286 1110 36 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-1.0865918689192569 2216 18 -1.1666983503279056 2216 19 -1.1358619329735191 2216 20 -1.0936772121530254 2216 21 -1.1651722559851478 2216 22 -1.1007295102037276 2216 23 -1.139801608654757 2216 24 -1.1596987693707703 2216 25 -1.1426207941016315 2216 26 -1.1421709624177154 2216 27 -1.158802484155089 2216 28 -1.1916278930852613 2216 29 -1.2270605475673548 2216 30 -1.2158320986579554 2216 31 -1.1995417119307135 2216 32 -1.1844901038546851 2216 33 -1.202866051491739 2216 34 -1.1946387942675756 2216 35 -1.212536582240702 2216 36 -1.2310971961563837 2216 37 -1.236639716491588 2216 38 -1.2595810757208312 2216 39 -1.2679759459042614 2216 40 -1.2473963495370128 2216 41 -1.3111749133822315 2216 42 -1.3252731755887481 2216 43 -1.2825553390947633 2216 44 -1.3040021221360831 2216 45 -1.3276600187073626 2216 46 -1.3732036485479426 2216 47 -1.3858539327349604 2216 48 -1.41784324468545 2216 49 -1.4037410892221516 2216 50 -1.401136681882143 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 575312.0 3 91902.0 4 159574.0 5 97891.0 6 75772.0 7 59996.0 8 52659.0 9 47877.0 10 45741.0 11 44270.0 12 44762.0 13 45960.0 14 47540.0 15 50655.0 16 54842.0 17 59725.0 18 65063.0 19 72266.0 20 82566.0 21 96933.0 22 114912.0 23 135365.0 24 156568.0 25 180280.0 26 206599.0 27 237870.0 28 275967.0 29 326159.0 30 392499.0 31 479447.0 32 596078.0 33 752188.0 34 991243.0 35 1359840.0 36 1947469.0 37 2795523.0 38 4532365.0 39 1.627811E7 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.02919848502503 18.938430903813753 13.193634063062387 25.83873654809884 2 16.472693410905983 19.79957193818016 37.85353919964013 25.874195451273728 3 17.571589714395937 23.078237347195806 32.22967855115559 27.120494387252663 4 12.655681921039763 18.4654003765947 39.61096037834078 29.267957324024763 5 15.206240145007435 36.51577369855804 35.082232901297914 13.19575325513661 6 30.755169902611417 38.428602799248125 18.689669664223608 12.126557633916855 7 29.20864253663843 31.556068282550047 21.96654001178061 17.268749169030915 8 26.454102060779427 33.11484773928084 21.48675959267482 18.944290607264914 9 26.940561369962364 15.419198023362801 20.427641745811382 37.21259886086346 10 15.335629312583453 25.819284143494937 34.150815377032494 24.694271166889116 11 34.77142818888196 21.975313417248472 25.02056719335542 18.232691200514143 12 23.8975890507 24.3250654960561 30.246058327989445 21.53128712525445 13 27.541574416011294 20.992905683001347 27.152822302560292 24.312697598427068 14 21.741067080924203 21.540058865012785 27.510108506945606 29.208765547117405 15 23.998825405884084 27.332752124824218 23.60819640841462 25.060226060877078 16 24.456163806093258 27.23862665106597 24.48428610910549 23.82092343373528 17 23.62577649984756 26.845013979072498 26.496418585101683 23.032790935978262 18 23.1216367795548 26.474194429821974 27.455242961045585 22.948925829577643 19 24.66390334758819 26.695708833880637 26.527229560186672 22.113158258344505 20 23.13535175973593 26.41240059960094 27.070470962854614 23.381776677808517 21 24.342824151466008 25.738635349604216 26.57177335781144 23.346767141118338 22 23.861236840093106 26.1706401446347 25.83620615791909 24.131916857353104 23 23.989466599384613 25.725105104419228 26.136151947051783 24.149276349144376 24 23.60397418072818 27.17021105663115 26.044567163904752 23.18124759873592 25 23.96599213386239 26.17445973946419 26.10942853700731 23.750119589666106 26 23.411545777344635 26.274728956369835 26.556616591836637 23.757108674448897 27 24.102150767699705 26.665771966874775 25.960605593007685 23.271471672417835 28 23.526122708975592 26.020126225818064 26.328766576620914 24.124984488585426 29 24.288790858290675 26.526073909038683 25.487609903393533 23.69752532927711 30 23.661298510280915 26.515984987446465 26.364276672593018 23.4584398296796 31 24.37948379084703 25.239601411736157 25.924893566656 24.456021230760815 32 23.026217834013817 25.9700975723417 26.68089620196149 24.322788391682995 33 24.24275080435271 25.270022447861557 26.531142709419925 23.956084038365805 34 23.96430301265554 26.108504772412928 26.609708045658724 23.317484169272806 35 24.532432081762128 26.24467465886279 25.866460536931957 23.356432722443127 36 23.815212575747207 25.95047334634799 25.955984099647743 24.27832997825706 37 24.66788001447595 25.881830542325073 24.944658688754636 24.505630754444336 38 24.19369841380009 25.632478383909895 26.653837637698253 23.51998556459176 39 24.035862367912173 25.966504840048117 25.41584349686339 24.58178929517632 40 23.80476362072953 25.840449947273292 26.987493025925506 23.367293406071667 41 23.77994912026253 26.084299020219504 26.176999846435358 23.958752013082613 42 23.57061961201497 26.215839361167493 26.205362377684636 24.0081786491329 43 23.797716029577654 25.553098578728502 26.46168602548245 24.187499366211394 44 24.17362123601507 25.633962593729116 26.086673113101266 24.105743057154548 45 23.926712369584852 25.652701714293595 26.48466682677319 23.93591908934836 46 24.092053763631764 26.026672457333756 25.940630257847353 23.94064352118713 47 23.86755634864833 25.6053146599039 26.457019775496 24.07010921595177 48 24.264410317711654 26.58501823545067 25.819340431257633 23.331231015580048 49 23.819087521610644 25.977650956613495 25.911435511711545 24.29182601006432 50 23.571207473105478 26.0017010445565 25.829907498825445 24.597183983512576 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 3978.0 1 3626.0 2 3274.0 3 2793.5 4 2313.0 5 2694.5 6 3076.0 7 3149.5 8 3223.0 9 3211.0 10 3199.0 11 4204.0 12 5209.0 13 7766.5 14 10324.0 15 19226.0 16 28128.0 17 58586.0 18 89044.0 19 109189.0 20 129334.0 21 178883.0 22 228432.0 23 254913.5 24 281395.0 25 399040.5 26 516686.0 27 707901.5 28 899117.0 29 984633.0 30 1070149.0 31 1002476.0 32 934803.0 33 987530.0 34 1040257.0 35 1156326.5 36 1272396.0 37 1493964.0 38 1715532.0 39 1792454.0 40 1869376.0 41 1955433.5 42 2041491.0 43 2017562.5 44 1993634.0 45 2016515.5 46 2039397.0 47 2074735.5 48 2110074.0 49 2181055.0 50 2252036.0 51 2188798.5 52 2125561.0 53 2029365.5 54 1933170.0 55 1842991.0 56 1752812.0 57 1671013.5 58 1589215.0 59 1459504.5 60 1329794.0 61 1161963.5 62 994133.0 63 871985.0 64 749837.0 65 631877.5 66 513918.0 67 481155.0 68 448392.0 69 429939.0 70 411486.0 71 346783.5 72 282081.0 73 281405.5 74 280730.0 75 211416.0 76 142102.0 77 128822.0 78 115542.0 79 117169.5 80 118797.0 81 106100.0 82 93403.0 83 87252.0 84 81101.0 85 66750.5 86 52400.0 87 50331.0 88 48262.0 89 31711.5 90 15161.0 91 8907.0 92 2653.0 93 2096.5 94 1540.0 95 1267.5 96 995.0 97 774.0 98 553.0 99 413.0 100 273.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06180235212901134 2 1.9625458239582122E-4 3 0.014305769634943878 4 0.0 5 0.021516638760850946 6 0.08022352088571001 7 0.05032740616741324 8 0.07110065635858305 9 0.03894761394273434 10 0.03448728252464749 11 0.060773502348572636 12 0.05872472345053142 13 0.05087454015469857 14 0.06455288983683156 15 0.04392534380531926 16 0.04727356592316312 17 0.057059533054445666 18 0.04532588787059853 19 0.03569157200753094 20 0.06175477526055175 21 0.031602934874284665 22 0.0155070855635486 23 0.012429456885068677 24 0.028174426790915245 25 0.013056876837879561 26 0.00866493716820338 27 0.0063069086251748 28 0.003945906527867496 29 0.011237061619300126 30 0.016042325333719024 31 0.009241806698275946 32 0.02429393845717969 33 0.0035533973630758543 34 0.004507908286546439 35 0.009125838081405687 36 0.013740794321986211 37 0.012979564426632723 38 0.004061875144737754 39 0.010930785528591497 40 0.004965835645470022 41 0.006708338452802617 42 0.010993230168444713 43 0.007865051067226472 44 0.005640832466740498 45 0.006470454110504651 46 0.009708654720035702 47 0.0016146399733474384 48 7.077059183364463E-4 49 0.005001518296814717 50 0.006586422727374908 >>END_MODULE >>Sequence Length Distribution pass #Length Count 50 3.3629788E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 39.31312469881424 #Duplication Level Percentage of deduplicated Percentage of total 1 74.25840649597741 29.193299945115974 2 15.851630409083665 12.463542459036436 3 4.847188051448004 5.716743249155335 4 1.8251505203995575 2.8700948001029403 5 0.8597239222278066 1.689921688054772 6 0.5190089648152638 1.2242318492150972 7 0.34900582395698565 0.9604356634483372 8 0.23472664231849 0.7382270207680612 9 0.1711818581204412 0.6056724361017268 >10 0.8962312801052921 6.483366486861337 >50 0.0839094690444456 2.2974468490137965 >100 0.07160437537955006 6.023798659779301 >500 0.013114039888176507 3.651781672673901 >1k 0.015836070201589978 13.31388607702453 >5k 0.0022057376831371924 5.981612769741824 >10k+ 0.0010763393504756302 6.785938373906558 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC 63188 0.18789294776404777 No Hit CTTTATTGGTGGCTGCTTTTAGGCCTACAATGGTTAAAAGCTGTTTTGTT 51851 0.15418176290614738 No Hit CTATTAAAGGTTTTTTCCGTTCCAGAAGAGCTGTCCCTCTTTTGGCTATA 49547 0.147330693847966 No Hit CCATAGGGTCTTCTCGTCTTATTATTATATTTCAGCCTCTTCACTGAAAG 48519 0.14427388004943711 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 2.973554278724564E-6 0.0 0.0 0.0 0.0 2 5.947108557449128E-6 0.0 0.0 5.947108557449128E-6 0.0 3 5.947108557449128E-6 0.0 0.0 2.3788434229796512E-5 0.0 4 5.947108557449128E-6 0.0 0.0 7.136530268938954E-5 0.0 5 5.947108557449128E-6 8.920662836173692E-6 0.0 1.1002150831280887E-4 0.0 6 1.1894217114898256E-5 1.1894217114898256E-5 0.0 1.1299506259153344E-4 2.973554278724564E-6 7 1.1894217114898256E-5 1.486777139362282E-5 0.0 1.6057193105112647E-4 2.973554278724564E-6 8 1.7841325672347384E-5 1.486777139362282E-5 0.0 1.7246614816602472E-4 2.973554278724564E-6 9 1.7841325672347384E-5 1.486777139362282E-5 0.0 3.389851877746003E-4 2.973554278724564E-6 10 2.081487995107195E-5 1.7841325672347384E-5 0.0 4.63874467481032E-4 2.973554278724564E-6 11 2.081487995107195E-5 1.7841325672347384E-5 0.0 5.292926616129724E-4 2.973554278724564E-6 12 2.081487995107195E-5 2.081487995107195E-5 0.0 6.48234832761955E-4 8.920662836173692E-6 13 2.3788434229796512E-5 2.081487995107195E-5 0.0 7.314943525662428E-4 1.1894217114898256E-5 14 2.3788434229796512E-5 2.081487995107195E-5 0.0 8.207009809279796E-4 1.1894217114898256E-5 15 2.3788434229796512E-5 2.3788434229796512E-5 0.0 0.0010972415288493641 1.7841325672347384E-5 16 2.6761988508521078E-5 2.3788434229796512E-5 0.0 0.0012518663513430414 1.7841325672347384E-5 17 2.6761988508521078E-5 2.3788434229796512E-5 0.0 0.0014927242479197311 2.3788434229796512E-5 18 2.6761988508521078E-5 2.6761988508521078E-5 0.0 0.0017365556987751455 2.973554278724564E-5 19 3.568265134469477E-5 2.6761988508521078E-5 0.0 0.0018436036528092297 2.973554278724564E-5 20 3.865620562341933E-5 2.973554278724564E-5 0.0 0.002126091309288063 2.973554278724564E-5 21 4.16297599021439E-5 2.973554278724564E-5 0.0 0.0023163987831264356 3.2709097065970206E-5 22 4.16297599021439E-5 2.973554278724564E-5 0.0 0.0026226748738350657 3.2709097065970206E-5 23 4.16297599021439E-5 2.973554278724564E-5 0.0 0.0029497658444947675 3.568265134469477E-5 24 4.7576868459593024E-5 2.973554278724564E-5 0.0 0.0033065923579417155 3.568265134469477E-5 25 5.649753129576672E-5 3.2709097065970206E-5 0.0 0.003725863511241879 3.568265134469477E-5 26 5.649753129576672E-5 3.2709097065970206E-5 0.0 0.004272997498527198 3.865620562341933E-5 27 5.649753129576672E-5 3.2709097065970206E-5 0.0 0.005254270410506304 3.865620562341933E-5 28 5.649753129576672E-5 3.2709097065970206E-5 0.0 0.008679804939597002 4.16297599021439E-5 29 6.244463985321585E-5 3.568265134469477E-5 0.0 0.013419650459883957 4.16297599021439E-5 30 6.541819413194041E-5 3.568265134469477E-5 0.0 0.01964329956525447 4.16297599021439E-5 31 6.839174841066497E-5 3.568265134469477E-5 0.0 0.033497088949832214 4.460331418086846E-5 32 6.839174841066497E-5 3.568265134469477E-5 0.0 0.049679171334651294 4.460331418086846E-5 33 6.839174841066497E-5 3.568265134469477E-5 0.0 0.06622105378719605 4.7576868459593024E-5 34 7.136530268938954E-5 3.568265134469477E-5 0.0 0.08656313860795078 5.0550422738317586E-5 35 8.920662836173692E-5 3.568265134469477E-5 0.0 0.1077021359754037 5.0550422738317586E-5 36 9.515373691918605E-5 3.568265134469477E-5 0.0 0.13613228843428926 5.0550422738317586E-5 37 9.515373691918605E-5 4.16297599021439E-5 0.0 0.1752345271995173 5.0550422738317586E-5 38 9.515373691918605E-5 4.16297599021439E-5 0.0 0.2265967302559267 5.3523977017042155E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGTTTGA 41240 0.0 27.18584 2 GTGTTTG 42745 0.0 26.892193 1 GTTTGAG 52940 0.0 20.465492 1 CGTACAT 4840 0.0 19.685398 19 TCGTACA 4865 0.0 19.53994 18 TTTGAGG 31880 0.0 19.371881 2 AATTCGT 5070 0.0 18.705654 15 AAATTCG 5155 0.0 18.441387 14 TTGAGGG 22150 0.0 17.537731 3 TTTGACG 4485 0.0 16.684572 4 CGTCTTA 14705 0.0 16.4002 15 GTATTGG 8095 0.0 15.478769 1 TCATACG 5395 0.0 15.042147 38 TAGGGTC 16500 0.0 14.912705 4 TCTCGTC 16520 0.0 14.718763 12 CATAGGG 17920 0.0 14.480324 2 ATAGGGT 17265 0.0 14.392287 3 CGTATCG 2615 0.0 14.139695 3 CCATAGG 18500 0.0 13.926922 1 GTCTTAT 17705 0.0 13.770591 16 >>END_MODULE