FastQCFastQC Report
Mon 6 Feb 2017
SRR2079134_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079134_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23663780
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN730670.30877146423775065No Hit
GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAACTAAAAGAATTACAGCTAGAAACCCC533580.22548384070507754No Hit
CTATTAAAGGTTTTTTCCGTTCCAGAAGAGCTGTCCCTCTTTTGGCTATAATCTAAACTTACTTTTTGATTTTGT410010.17326479539617085No Hit
CTTTATTGGTGGCTGCTTTTAGGCCTACAATGGTTAAAAGCTGTTTTGTTAATTATTCACTATTAAAGGTTTTTT393460.16627098460178383No Hit
CCATAGGGTCTTCTCGTCTTATTATTATATTTCAGCCTCTTCACTGAAAGGTCAATTTCACTGATTAAAGATAAG274170.11586061060405396No Hit
CCTCTAGATGGATATAAAGTACCGCCAAGTCCTTTGAGTTTTAAGCTATGGCTAGTAGTTCTCTGGCAAATAGTT273130.11542112037890818No Hit
CTCTTAGGGTTGGTAAATTTCGTGCCAGCCACCGCGGTCATACGATTAACCCAAACTAATTATCTTCGGCGTAAA259640.1097204250546616No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTTGA423600.036.977042
GTGTTTG442850.036.525781
GTTTGAG458650.027.936451
TTTGACG40950.024.2598484
TTTGAGG298350.024.1399482
TTGAGGG204550.021.7342433
CGTCTTA105700.021.49726515
TCTCGTC119650.019.10313612
GCGTAAA104550.018.15864869
AAATTCG49150.018.11182214
GGCGTAA105050.018.06960168
TAATGTG141650.017.9855695
TAGGGTC130850.017.9524144
TCGTACA49350.017.81078118
CGTACAT49150.017.74901219
TTGAGTG119550.017.580345
ACGACCG68750.017.49504717
AATTCGT51550.017.20147315
AATGTGC138300.017.0465096
ATAGGGT142300.017.0146623
Produced by FastQC (version 0.11.5)