FastQCFastQC Report
Mon 6 Feb 2017
SRR2079134_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079134_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23663780
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAACTAAAAGAATTACAGCTAGAAACCCC527110.2227497044005649No Hit
CTATTAAAGGTTTTTTCCGTTCCAGAAGAGCTGTCCCTCTTTTGGCTATAATCTAAACTTACTTTTTGATTTTGT439320.18565081318369256No Hit
CTTTATTGGTGGCTGCTTTTAGGCCTACAATGGTTAAAAGCTGTTTTGTTAATTATTCACTATTAAAGGTTTTTT423780.17908381501180287No Hit
CCATAGGGTCTTCTCGTCTTATTATTATATTTCAGCCTCTTCACTGAAAGGTCAATTTCACTGATTAAAGATAAG305070.12891854133194275No Hit
CCTCTAGATGGATATAAAGTACCGCCAAGTCCTTTGAGTTTTAAGCTATGGCTAGTAGTTCTCTGGCAAATAGTT291560.1232093942725972No Hit
CCCTAAGAGGTATAACTTAGTCAAACTTTCGTTTATTGCTTAATATTTATCACTGCTGAGTCCCGTGGGGGTGTG269020.1136842888160725No Hit
CTCTTAGGGTTGGTAAATTTCGTGCCAGCCACCGCGGTCATACGATTAACCCAAACTAATTATCTTCGGCGTAAA268050.11327437966377307No Hit
ACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAACTAAAAGAATTACAGCTAGAAACCCCGAAACC246960.10436202500192276No Hit
GCATAGTGGGGTATCTAATCCCAGTTTGGGTCTTAGCTGTCGTGTATTATAAATGACTAGAATTACTTTCGTTAT245920.103922534776777No Hit
AATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAGTTTAAAATTGAACTTAAATTCATTTTTTGGGTAA237230.10025025587628011No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTTGA371150.033.4087452
GTGTTTG384300.033.0411721
GTTTGAG399900.027.0891461
TTTGAGG274000.022.8790782
CGTCTTA103000.021.80344215
TTGAGGG183600.021.6171443
ACGACCG67900.019.50951417
TCTCGTC118450.019.10719312
TAGGGTC126150.018.2204574
GGCGTAA109550.018.0751868
GCGTAAA110400.017.9667369
GTCGGAC74750.017.9542212
ACCGATT72950.017.92440620
CCGATTT74050.017.79795521
TTTGACG32850.017.650064
TCGGACG74750.017.63113613
ACCGCTA74900.017.36129638
TATTGGT200300.017.281934
ATAGGGT139500.016.9716133
TACGGAC74700.016.94875543
Produced by FastQC (version 0.11.5)