FastQCFastQC Report
Mon 6 Feb 2017
SRR2078272_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2078272_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2364506
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA42750.037.91981
GGTATCA21250.034.2474141
ATCAACG56250.027.713583
TCAACGC56400.027.6404574
TATCAAC58650.027.3998782
CAACGCA57600.026.943155
AACGCAG61150.025.4219286
ACGCAGA70900.021.733347
CGCAGAG73050.020.8612738
AGAGTAC71750.019.61168911
GTACATG81750.019.2811411
GAGTACT31150.018.93279612
GTACTAG3500.018.7236211
TGCGTAG1852.655106E-718.64253414
TACATGG83600.018.438772
CAGAGTA78000.018.43818510
GCAGAGT79100.018.3997829
GTGGTAT11100.018.332991
TATACCG950.0078535818.1511735
ACATGGG86200.017.6844563
Produced by FastQC (version 0.11.5)