FastQCFastQC Report
Mon 6 Feb 2017
SRR2078272_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2078272_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2364506
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31220.13203603627988258No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26500.044.4770431
GTATCAA64600.040.6761251
TATCAAC91500.028.4489572
ATCAACG93900.027.6468243
TCAACGC94850.027.2602944
CAACGCA96750.026.724955
AACGCAG100300.025.8136086
CGTGCCG2700.022.99573310
ACGCAGA117800.021.786577
GTGGTAT9000.021.5699141
CGCAGAG120050.021.3183068
GAGTACT79900.021.24011412
CGCGATC1304.9424198E-621.22234223
AGAGTAC118400.020.62624211
AGTACTT83500.020.07651513
GTACATG78500.019.828031
CGACCCG1055.840629E-419.8057375
GCAGAGT126150.019.7397929
CAGAGTA125300.019.62804410
GTACTTT86050.019.44148414
Produced by FastQC (version 0.11.5)