FastQCFastQC Report
Mon 6 Feb 2017
SRR2078271_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2078271_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2636599
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20400.033.971731
GTATCAA38050.030.4464281
TCAACGC47400.024.363744
ATCAACG48650.023.8072473
CAACGCA48600.023.7612655
TATCAAC49850.023.3711222
TATACCG902.1392794E-422.9810455
AACGCAG50800.022.5905156
GTACATG76650.021.6363851
TACATGG76600.021.2843512
CGTGCCG3800.019.9625310
ACATGGG80050.019.937583
ACGCAGA58700.019.1399867
GTGGTAT12250.018.2948571
GTATAAT8450.017.953441
CGCAGAG62100.017.7052638
CATGGGG60650.017.2790684
AGAGTAC58200.017.12187611
GAGTACT23750.016.69593412
TGGTATC12750.016.4910262
Produced by FastQC (version 0.11.5)