FastQCFastQC Report
Mon 6 Feb 2017
SRR2078271_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2078271_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2636599
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22150.042.5848961
GTATCAA54650.040.230881
TATCAAC74800.028.967192
ATCAACG77000.028.4059013
TCAACGC77100.028.3241024
CAACGCA78350.027.7394925
AACGCAG81800.026.6966786
ACGCAGA95850.022.6659857
CGGCACG3150.021.89739622
CGCAGAG99750.021.6495978
AGAGTAC96550.020.6505211
GAGTACT62550.020.34953712
GTACATG70150.020.16941
CGATTCG2102.3701432E-919.70242546
CAGAGTA104600.019.48997310
CGTGCCG4450.019.3791810
GCAGAGT107400.019.2395299
TACATGG72450.019.2360342
AGTACTT67700.018.75057613
ACATGGG74650.018.2487343
Produced by FastQC (version 0.11.5)