FastQCFastQC Report
Mon 6 Feb 2017
SRR2078270_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2078270_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2516605
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16450.035.8403051
GTATCAA33500.034.0662771
ATCAACG42500.026.7668553
TCAACGC42400.026.6679124
CAACGCA43450.026.1002085
TATCAAC44250.025.6288492
AACGCAG44450.025.583966
TCGCAAT2650.022.13687149
CGTGCCG3350.021.61648810
ACGCAGA53200.021.2473627
CGCAGAG55800.020.1991968
AGAGTAC54450.019.18913511
CGCCCAA4050.018.74965750
GCACCGT5150.018.7359776
GTACATG59650.018.6695371
CAATCGC3150.018.62827746
GAGTACT27000.018.26345412
TCAATCG3250.018.03677645
CAGAGTA59950.018.00386610
GTGCCGA4250.017.85025411
Produced by FastQC (version 0.11.5)