FastQCFastQC Report
Mon 6 Feb 2017
SRR2078270_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2078270_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2516605
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21100.042.8631741
GTATCAA47050.036.4562381
ATCAACG65750.026.076243
TATCAAC65050.025.9882742
CAACGCA67150.025.429425
TCAACGC67350.025.4053334
AACGCAG70350.024.420426
TGCGATA3450.022.99927713
GCGATAC3550.022.35141214
TTGTGCG3100.021.13567558
ACCGTAT2301.4551915E-1120.9980898
ACGCAGA81850.020.5899727
GTACATG60200.020.5493451
CGCAGAG83750.020.2244268
CGAATCG3200.019.39561737
TACATGG60850.019.1855472
ACCGCGT900.005739290319.1649238
CGGCACG3500.018.7242622
AGAGTAC83600.018.5699911
GAGTACT55400.018.37036112
Produced by FastQC (version 0.11.5)