FastQCFastQC Report
Mon 6 Feb 2017
SRR2078269_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2078269_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1846743
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12550.035.7157481
GTATCAA24550.033.706981
TCAACGC30850.026.4861014
ATCAACG31800.025.8032673
CAACGCA31650.025.70635
TATCAAC32750.025.0534172
AACGCAG33050.024.8165826
CGTGCCG2750.021.31860510
GTACATG47200.021.03831
TACATGG48250.020.6491322
ACGCAGA38900.020.643257
CGCAGAG40900.019.5521878
ACTCGAC2153.1304808E-919.26575346
AGAGTAC38350.019.15388111
ACATGGG51600.018.975513
TACATCG1852.6656198E-718.6350085
GTCCGTA950.00786318618.1470721
AGCACCG5200.017.9003585
GAGTACT19100.017.87493112
GTGCCGA3300.017.76550711
Produced by FastQC (version 0.11.5)