FastQCFastQC Report
Mon 6 Feb 2017
SRR2078269_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2078269_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1846743
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15500.039.8340071
GTATCAA35950.038.980571
ACGCGTT450.00463450230.65164867
ATCAACG51150.027.5838153
TCAACGC51250.027.5299914
TATCAAC51150.027.3164392
CAACGCA52200.026.7633275
AACGCAG54550.025.5468226
GCGATAC2250.024.5279614
ACGCAGA63500.021.7540367
CGCAGAG66950.020.6078838
GAGTACT40800.020.54329312
ATGCGCG850.004134114420.28308151
AGAGTAC64400.020.08542811
TACCGTA900.00570264319.1858527
CAGAGTA69100.019.16853310
TTACGTC900.005753762619.15624238
GTACATG51150.018.8523241
CGAATCG2204.2218744E-918.80743837
AGTACTT44700.018.67376113
Produced by FastQC (version 0.11.5)