FastQCFastQC Report
Mon 6 Feb 2017
SRR2078268_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2078268_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1714667
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12550.036.5500031
GTATCAA22500.032.189651
TCAACGC29050.024.9302884
ATCAACG29000.024.854353
CAACGCA29550.024.5063085
TATCAAC29400.024.2787552
AACGCAG30850.023.799936
TAGATCG750.001998843622.9892525
GTACATG47100.021.3816621
ACGCAGA36000.020.0151317
TTGCGCG1056.024639E-419.71428753
TACATGG49800.019.4572032
CGTACAC2354.620233E-1019.077863
AGAGTAC36400.018.94884711
TAATTCG1108.240587E-418.81763359
ACATGGG49300.018.8173963
CGCAGAG38050.018.6666018
CGTGCCG2801.2732926E-1118.47512410
GTGCCGA2651.1641532E-1018.21949411
ACCGTCG1351.5849322E-417.8779188
Produced by FastQC (version 0.11.5)