FastQCFastQC Report
Mon 6 Feb 2017
SRR2078268_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2078268_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1714667
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13400.041.6560671
GTATCAA29400.038.7976841
TCAACGC40100.027.564424
TATCAAC41050.027.3533692
ATCAACG41650.026.7053
CAACGCA41400.026.6988725
AACGCAG42800.025.9874616
GTACATG45350.022.3235261
ACGCAGA49750.022.0795177
TACATGG46600.021.1952172
CGCAGAG52600.020.7914038
AGAGTAC50750.020.66498811
ACATGGG47600.020.0911463
CAGAGTA54550.019.54165510
GAGTACT34650.019.41462512
GTACTTT37000.018.64772614
TACACCG2255.2477844E-918.479975
AGTACTT36800.018.46783613
GCAGAGT58600.018.3072199
CATGGGG35000.018.2159734
Produced by FastQC (version 0.11.5)