FastQCFastQC Report
Mon 6 Feb 2017
SRR2078267_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2078267_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2305442
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17100.037.3067471
GTATCAA33950.035.4484631
CAACGCA45400.026.2741245
TCAACGC45900.026.214384
ATCAACG46050.026.0529943
TATCAAC46950.025.845612
AACGCAG48100.024.7136486
TGCGATA3300.021.94549613
ACGCAGA55600.021.3217057
GCGATAC3400.021.30004114
GTACATG64800.021.0200561
CGCAGAG58050.020.0097378
TATACCG1056.0546474E-419.7001675
TACATGG66300.019.6543162
AGAGTAC55500.019.26232711
ATACCGT2002.9269358E-818.950716
ACATGGG70700.018.4323373
GAGTACT26200.018.16426712
CAGAGTA60700.018.00987210
GTGCGAT4450.017.82411212
Produced by FastQC (version 0.11.5)