FastQCFastQC Report
Mon 6 Feb 2017
SRR2078267_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2078267_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2305442
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18100.043.8694841
GTATCAA43850.039.6953621
TATCAAC62600.027.4090822
CAACGCA63900.027.0112535
ATCAACG64400.026.8553543
TCAACGC64550.026.6855644
AACGCAG67900.025.4200176
CGCGTAA851.4493614E-424.3394365
ACGCAGA80400.021.2297447
GCGATAC2950.021.05058314
GAGTACT50000.020.83728612
CGCAGAG83250.020.3430778
TGCGATA2950.019.88110513
AGAGTAC79800.019.80007611
AGTACTT53350.019.6586113
GTACTTT54700.018.9842214
CAGAGTA86750.018.7307710
TCGCAAT2801.2732926E-1118.47148749
GTACATG61700.018.3205321
TCGCGTA1150.001115151817.98923364
Produced by FastQC (version 0.11.5)