FastQCFastQC Report
Mon 6 Feb 2017
SRR2078266_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2078266_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1357044
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA18100.034.2972951
GGTATCA9000.029.8894581
TCAACGC21400.027.8782964
CAACGCA21500.027.2664325
ATCAACG21900.026.9248853
AACGCAG22550.026.13726
TATCAAC23250.026.1001782
GTGCGAT1951.8189894E-1224.754112
TGCGATA1851.8189894E-1124.22843413
GGTGCGA1953.8198777E-1122.9859511
GCGATAC1953.8198777E-1122.9859514
ACGCAGA25800.022.1775677
GTACATG31100.021.73511
CGCAGAG27800.020.584348
TACATGG32300.020.1749762
AGAGTAC26500.020.1669211
CGAATCG1206.2971645E-520.16256137
CACGTCC2303.4197E-1019.51257347
ATAGGCG1601.3648623E-619.40227356
TAACCCG900.00575030919.1577875
Produced by FastQC (version 0.11.5)