FastQCFastQC Report
Mon 6 Feb 2017
SRR2078266_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2078266_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1357044
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA24200.041.4251331
GGTATCA9400.038.0093841
CAACGCA35050.028.6857415
ATCAACG35300.028.58083
TCAACGC35450.028.3620664
AACGCAG36900.026.9656946
TATCAAC36850.026.912192
CGTGCCG1951.8189894E-1224.76478410
GTACATG33450.022.9180471
ACGCAGA44200.022.195357
TACATGG34150.021.5261122
GTGCCGA2600.021.22695711
GAGTACT31300.021.04816212
CGCAGAG46500.020.9930068
GCACCGT3200.020.5854196
GCGATAC1701.08775566E-720.28972814
AGAGTAC45750.020.2062111
ACATGGG35700.020.1999933
AGTACTT34200.019.46508613
GTGCGAT1952.1605956E-819.45732712
Produced by FastQC (version 0.11.5)